What can you do with Iconeus One?

Unravelling intrinsic brain function and network organization is a major challenge for neuroscientists today.

To date, most scientific tools available to observe brain activity are either limited by poor sensitivity or a small field of view, hindering you from seeing the big picture. 

Functional ultrasound (fUS) is a revolution for researchers who wish to embrace a more holistic approach.

Adding whole-brain functional imaging to your experimental read-outs has never been easier thanks to Iconeus One, the first and only commercial fUS system, currently the most powerful and easy-to-use tool available for investigating neurovascular structure as well as brain activation and connectivity. 

 

 

Quantitative measurement of cerebral blood volume (CBV)

Abnormalities in CBV have been reported in many brain disorders, including stroke, Huntington's desease, Parkinson's and Alzheimer’s. With Iconeus One and its powerful software suite, you can easily assess the temporal evolution of CBV in the whole brain in a completely non-invasive manner.

Functional ultrasound (fUS) imaging uses ultra-high sensitivity power Doppler sequences to estimate the CBV in small vessels and detect hemodynamic events such as dilation and vasoconstriction with unmatched temporal and spatial resolution.

Power Doppler images obtained from an automatic scan of a rat's brain. Voxel intensity is directly proportional to cerebral blood volume (CBV).

Pharmaco-fUS by Iconeus One is the perfect solution in CNS drug development for identifying the central mechanism of action of new lead compounds. By directly modulating neuronal activity, centrally acting drugs can cause local changes in CBV, in brain areas expressing their target receptors.

With functional ultrasound, you can study the regional selectivity of non-vasoactive drugs and establish essential PK/PD parameters such as dose-response relationships in a non-invasive manner. Iconeus One includes an automatic 3D Brain Atlas registration onto vascular images to help you reliably define your functional regions of interest.

Effect of a centrally acting compound on CBV time course in an anesthetized rat. Changes in CBV are restricted to cortical areas.

Functional neuroimaging

When neurons are activated, their need for energy and oxygen drives an increase of local blood flow. This phenomenon is called neurovascular coupling and is tightly regulated in the mammalian brain. With fUS, you can use CBV as a surrogate for neuronal activity with a much better sensitivity and spatio-temporal resolution than the BOLD response measured with fMRI.

During a task, CBV typically increases between 10-50% in activated regions.

Activations can be detected within a single trial, with no need for averaging a large number of stimulations. 

Transcranial imaging of the mouse brain during whisker stimulation, Jeremy Ferrier, Iconeus One

3D fUS volumes of the rodent brain

Iconeus One maps 3D brain activation and intrinsic connectivity patterns with ultra-high sensitivity and resolution. For more information, read our newsletter on the state-of-the art in multidimensional fUS imaging.

Resting-state Functional Connectivity (rsFC)

Functional connectivity is defined as the temporal correlation between distinct neurophysiological events in the brain. By looking at spontaneous brain activity, rsFC can help to reveal the functional architecture of the brain by identifying and weighting intrinsic brain networks.

Functional connectivity has been shown to be related to structural wiring between brain regions and has been consistently reported to be impaired in neuropsychiatric disorders, even before any clinical symptoms. 

Correlation matrix of resting-state connectivity obtained across 45 ROIs at Bregma -1.2 mm in an anesthetized mouse.

 

With Iconeus, mapping the brain connectome has never been easier.

Iconeus One includes an automatic 3D brain atlas registration onto vascular images to help you reliably define your functional regions of interest.

Correlation matrices can be generated within a few steps to identify pathological biomarkers in a quantitative manner.

Functional resting-state networks (RSNs) can be revealed and visualized through the intact skull in mice by a seed-based approach with a simple click of the mouse.

Thanks to our motorized scanning stage (XYZ translation + R rotation), you can perform multi-slice imaging of the brain with high accuracy and repeatability.

The whole brain of a mouse can be imaged within a few seconds.

Multi-slice functional connectivity matrix obtained in an anesthetized mouse.

Blood vessel mapping

As brain function relies closely on bloodstream supply of oxygen and nutrients, the study of the vascular system in health and disease is a crucial requirement in neuroscience. Alterations of the vascular structures have been found in a multitude of brain pathologies, including Alzheimer’s disease and stroke.

With functional ultrasound, you can visualize the whole vascular tree and assess anatomical variations in pathological models. Iconeus provides a complete solution for acquiring isometric 3D volumes of the whole brain vascularization. Applications include stroke monitoring (ischemia/reperfusion), aneurysm detection and vascular plasticity (angiogenesis).

Whole-brain vascularization structure obtained in an anesthetized rat. Reconstructed tomographic scan (total acquisition time ~ 30 min)
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